Suggested by Flavio

National Yang-Ming University, Taipei, Taiwan

The materials are prepared for the Next-Generation Sequencing Workshop organized by the Life Science Library Training Courses on 19th Nov 2015 at Academia Sinica. [ PDF File ]

As the title suggests, RNA-Seq (RNA sequencing) utilizes the next-generation technology to assay the presence and quantity of RNA molecules in the given sample. Unlike the microarray technology that has been used since 2000s, RNA-Seq offers many advantages. This includes detects known and novel transcripts, increased specificity and sensitivity, and identification of low-abundance transcripts and isoforms with sufficient sequencing depth. RNA-seq has also been used to discover alternative splicing variants, chimeric RNAs result from fusion genes and RNA editing sites.