Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.
Antigen-specific B cells bifurcate into antibody-secreting cells (ASCs) and memory B cells (MBCs) after infection or vaccination. ASCs (plasmablasts) have been extensively studied in humans, but less is known about B cells that become activated but do not differentiate into plasmablasts. Here we have defined the phenotype and transcriptional program of a subset of antigen-specific B cells, which we have called 'activated B cells' (ABCs), that were distinct from ASCs and were committed to the MBC lineage. We detected ABCs in humans after infection with Ebola virus or influenza virus and also after vaccination. By simultaneously analyzing antigen-specific ASCs and ABCs in human blood after vaccination against influenza virus, we investigated the clonal overlap and extent of somatic hypermutation (SHM) in the ASC (effector) and ABC (memory) lineages. Longitudinal tracking of vaccination-induced hemagglutinin (HA)-specific clones revealed no overall increase in SHM over time, which suggested that repeated annual immunization might have limitations in enhancing the quality of influenza-virus-specific antibody.
Authors
Ali H Ellebedy; Katherine J L Jackson; Haydn T Kissick; Helder I Nakaya; Carl W Davis; Krishna M Roskin; Anita K McElroy; Christine M Oshansky; Rivka Elbein; Shine Thomas; George M Lyon; Christina F Spiropoulou; Aneesh K Mehta; Paul G Thomas; Scott D Boyd; Rafi Ahmed
External link
Publication Year
Publication Journal
Associeted Project
Microbiology or Immunology
Lista de serviços
-
Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells.Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells.
-
RNA-Binding Protein Musashi1 Is a Central Regulator of Adhesion Pathways in Glioblastoma.RNA-Binding Protein Musashi1 Is a Central Regulator of Adhesion Pathways in Glioblastoma.
-
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes.MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes.
-
Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription.Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription.
-
Antimicrobial peptide LL-37 participates in the transcriptional regulation of melanoma cells.Antimicrobial peptide LL-37 participates in the transcriptional regulation of melanoma cells.
-
Down-regulation of 14q32-encoded miRNAs and tumor suppressor role for miR-654-3p in papillary thyroid cancer.Down-regulation of 14q32-encoded miRNAs and tumor suppressor role for miR-654-3p in papillary thyroid cancer.
-
Integration of miRNA and gene expression profiles suggest a role for miRNAs in the pathobiological processes of acute Trypanosoma cruzi infection.Integration of miRNA and gene expression profiles suggest a role for miRNAs in the pathobiological processes of acute Trypanosoma cruzi infection.
-
Integrative Biology Approaches Applied to Human DiseasesIntegrative Biology Approaches Applied to Human Diseases
-
Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock.Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock.
-
Genomics, epigenomics and pharmacogenomics of Familial Hypercholesterolemia (FHBGEP): A study protocol.Genomics, epigenomics and pharmacogenomics of Familial Hypercholesterolemia (FHBGEP): A study protocol.
-
Melatonin-Index as a biomarker for predicting the distribution of presymptomatic and asymptomatic SARS-CoV-2 carriersMelatonin-Index as a biomarker for predicting the distribution of presymptomatic and asymptomatic SARS-CoV-2 carriers
-
Profiling plasma-extracellular vesicle proteins and microRNAs in diabetes onset in middle-aged male participants in the ELSA-Brasil study.Profiling plasma-extracellular vesicle proteins and microRNAs in diabetes onset in middle-aged male participants in the ELSA-Brasil study.
-
Big Data and machine learning in cancer theranosticsBig Data and machine learning in cancer theranostics
-
Genomic positional conservation identifies topological anchor point RNAs linked to developmental loci.Genomic positional conservation identifies topological anchor point RNAs linked to developmental loci.
-
Integrative systems immunology uncovers molecular networks of the cell cycle that stratify COVID-19 severityIntegrative systems immunology uncovers molecular networks of the cell cycle that stratify COVID-19 severity