TLR5-mediated sensing of gut microbiota is necessary for antibody responses to seasonal influenza vaccination.
Systems biological analysis of immunity to the trivalent inactivated influenza vaccine (TIV) in humans revealed a correlation between early expression of TLR5 and the magnitude of the antibody response. Vaccination of Trl5(-/-) mice resulted in reduced antibody titers and lower frequencies of plasma cells, demonstrating a role for TLR5 in immunity to TIV. This was due to a failure to sense host microbiota. Thus, antibody responses in germ-free or antibiotic-treated mice were impaired, but restored by oral reconstitution with a flagellated, but not aflagellated, strain of E. coli. TLR5-mediated sensing of flagellin promoted plasma cell differentiation directly and by stimulating lymph node macrophages to produce plasma cell growth factors. Finally, TLR5-mediated sensing of the microbiota also impacted antibody responses to the inactivated polio vaccine, but not to adjuvanted vaccines or the live-attenuated yellow fever vaccine. These results reveal an unappreciated role for gut microbiota in promoting immunity to vaccination.
Authors
Jason Z Oh; Rajesh Ravindran; Benoit Chassaing; Frederic A Carvalho; Mohan S Maddur; Maureen Bower; Paul Hakimpour; Kiran P Gill; Helder I Nakaya; Felix Yarovinsky; R Balfour Sartor; Andrew T Gewirtz; Bali Pulendran
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Associeted Project
Microbiology or Immunology
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