HF diet increased total serum HDL and LDL, as well as vascular AhR protein expression and proinflammatory cytokines in the adipose tissue. HF diet decreased endothelium-dependent vasodilation. AhR deletion protected mice from HF diet-induced dyslipidaemia, weight gain and inflammatory processes. HF diet-induced endothelial dysfunction was attenuated in Ahr-/- mice. Vessels from Ahr-/- mice exhibited a greater NO reserve. In cultured endothelial cells, lysophosphatidylcholine (LPC) a major component of LDL and oxidized LDL [oxLDL]) reduced Nos3 gene expression and NO production. Antagonism of the AhR inhibited LPC effects on endothelial cells and induced decreased endothelium-dependent vasodilation.
da Silva, Josiane Fernandes; Bolsoni, Juliana A; da Costa, Rafael M; Alves, Juliano V; Bressan, Alecsander FM; Silva, Luiz Eduardo V; Costa, Tiago J; Oliveira, Antonio ER; Manzato, Carla P; Aguiar, Carlos A;
Microbiology or Immunology